Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Total Antioxidant Capacity (also known as T-AOC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat T-AOC antibody. T-AOC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat T-AOC Antibody is added and binds to T-AOC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated T-AOC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat T-AOC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm